yitJ [2018-04-02 14:59:20]

methionine synthase

locus: BSU11010

pI: 5.34

mw: 67.75 kDa

protein length: 612 aa

gene length: 1836 bp

function: methionine biosynthesis

product: methionine synthase

essential: no

ec: 2.1.1.13

synonyms

Genomic Context

categories

• [category|SW 2|Metabolism] → [category|SW 2.3|Amino acid/ nitrogen metabolism] → [category|SW 2.3.1|Biosynthesis/ acquisition of amino acids] → [category|SW 2.3.1.11|Biosynthesis/ acquisition of methionine/ S-adenosylmethionine/ based on similarity]
• [category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins]

Gene

Coordinates: 1,178,757 → 1,180,595

The protein

[SW|Localization]

• membrane associated [Pubmed|18763711]

Expression and Regulation

Operons

• genes: [gene|41AB43191DDB2E0698D92FFD1ECEF45100F1F3F0|yitJ]

  description

  • [Pubmed|10094622]

  regulatory mechanism

  • [regulon|S-box|S-box]: termination/antitermination, [pubmed|10094622], in [regulon|S-box|S-box]

  regulation

  • induced by methionine starvation ([SW|S-box]) [Pubmed|10094622]
  • the [SW|S-box] RNA is degraded by [protein|872CCB5A49C9000BD95E4B0472556D5F60F7D7A4|RNase Y] [pubmed|29794222]

Biological materials

Mutant

• MGNA-B195 (yitJ::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1194 NBRP B. subtilis, Japan]
• BKE11010 (Δ[gene|41AB43191DDB2E0698D92FFD1ECEF45100F1F3F0|yitJ]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE11010 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTGTCTGCCTCCTTTATT, downstream forward: _UP4_TAATTTCCAAAAGACTGCCT
• BKK11010 (Δ[gene|41AB43191DDB2E0698D92FFD1ECEF45100F1F3F0|yitJ]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK11010 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTGTCTGCCTCCTTTATT, downstream forward: _UP4_TAATTTCCAAAAGACTGCCT

References

The [protein|search|S-box]

• 19258532,10094622,18039762,20951706,22543867

Other publications

• 19779461,12107147,18763711,22960854